James L. Funderburgh, PhD

  • Professor of Ophthalmology
  • Professor, Cell Biology & Physiology
  • Associate Director, Fox Center for Vision Restoration
  • University of Pittsburgh School of Medicine

Academic Affiliation

1973-1975: Assistante de Recherche (Post Doctoral), Department of Pathology, University of Geneva, Ge-neva, Switzerland

1975-1978: Senior Fellow, Dept. of Ophthalmology, University of Washington, Seattle, WA

1978-1983: Research Associate, Corneal Disease Research Laboratory, Swedish Hospital Medical Center, Seattle, WA

1983-1999 Research Professor, Division of Biology Kansas State University

1999-present: Professor, Department of Ophthalmology University of Pittsburgh,Pittsburgh, PA

1999-present Professor, Department of Cell biology and Physiology University of Pittsburgh,Pittsburgh, PA

2001-present Member, McGowan Institute for Regenerative Medicine, University of Pittsburgh,Pittsburgh, PA

Education & Training

  • University of Texas at Austin, Austin, TX, B.A. Chemistry
  • University of Minnesota, M.S. Physiological Chemistry
  • University of Wisconsin, Madison, PhD Biological Chemistry

Representative Publications

  1. The engineering of organized human corneal tissue through the spatial guidance of corneal stromal stem cells. Wu, J., Y. Du, S. C. Watkins, J. L. Funderburgh and W. R. Wagner. Biomaterials (2012) 33(5): 1343-1352, PMCID 3254093.
  2. Concise review: Stem cells in the corneal stroma. Pinnamaneni, N. and J. L. Funderburgh. Stem cells (2012) 30(6): 1059-1063, 
  3. Multipotent stem cells from trabecular meshwork become phagocytic TM cells. Du, Y., D. S. Roh, M. M. Mann, M. L. Funderburgh, J. L. Funderburgh and J. S. Schuman. Investigative ophthalmology & visual science (2012) 53(3): 1566-1575, PMCID 3339918.
  4. Quantitative assessment of ultrastructure and light scatter in mouse corneal debridement wounds. Boote, C., Y. Du, S. Morgan, J. Harris, C. S. Kamma-Lorger, S. Hayes, K. L. Lathrop, D. S. Roh, M. K. Burrow, J. Hiller, N. J. Terrill, J. L. Funderburgh and K. M. Meek. Investigative ophthalmology & visual science (2012) 53(6): 2786-2795, PMCID 3367468.
  5. Rapid changes in connexin-43 in response to genotoxic stress stabilize cell-cell communication in corneal endothelium. Roh, D. S. and J. L. Funderburgh. Investigative ophthalmology & visual science (2011) 52(8): 5174-5182, PMCID 3176070.
  6. Lumican is required for neutrophil extravasation following corneal injury and wound healing. Hayashi, Y., M. K. Call, T. Chikama, H. Liu, E. C. Carlson, Y. Sun, E. Pearlman, J. L. Funderburgh, G. Babcock, C. Y. Liu, Y. Ohashi and W. W. Kao. Journal of cell science (2010) 123(Pt 17): 2987-2995, PMCID 2923572.
  7. Hyaluronan synthesis mediates the fibrotic response of keratocytes to transforming growth factor beta. Guo, N., X. Li, M. M. Mann, M. L. Funderburgh, Y. Du and J. L. Funderburgh. The Journal of biological chemistry (2010) 285(42): 32012-32019, PMCID 2952202.
  8. Adipose-derived stem cells differentiate to keratocytes in vitro. Du, Y., D. S. Roh, M. L. Funderburgh, M. M. Mann, K. G. Marra, J. P. Rubin, X. Li and J. L. Funderburgh. Molecular vision (2010) 16: 2680-2689, PMCID 3002955.
  9. Stromal edema in klf4 conditional null mouse cornea is associated with altered collagen fibril organization and reduced proteoglycans. Young, R. D., S. K. Swamynathan, C. Boote, M. Mann, A. J. Quantock, J. Piatigorsky, J. L. Funderburgh and K. M. Meek. Investigative ophthalmology & visual science (2009) 50(9): 4155-4161, PMCID 2761629.
  10. Impact on the corneal endothelium of mitomycin C during photorefractive keratectomy. Roh, D. S. and J. L. Funderburgh. Journal of refractive surgery (2009) 25(10): 894-897, PMCID 2876307.
  11. Stem cell therapy restores transparency to defective murine corneas. Du, Y., E. C. Carlson, M. L. Funderburgh, D. E. Birk, E. Pearlman, N. Guo, W. W. Kao and J. L. Funderburgh. Stem cells (2009) 27(7): 1635-1642, PMCID 2877374.
  12. DNA cross-linking, double-strand breaks, and apoptosis in corneal endothelial cells after a single exposure to mitomycin C. Roh, D. S., A. L. Cook, S. S. Rhee, A. Joshi, R. Kowalski, D. K. Dhaliwal and J. L. Funderburgh. Investigative ophthalmology & visual science (2008) 49(11): 4837-4843, PMCID 2875544.
  13. Keratocyte phenotype is enhanced in the absence of attachment to the substratum. Funderburgh, M. L., M. M. Mann and J. L. Funderburgh. Molecular vision (2008) 14: 308-317, PMCID 2255023.
  14. A rapid transient increase in hyaluronan synthase-2 mRNA initiates secretion of hyaluronan by corneal keratocytes in response to transforming growth factor beta. Guo, N., D. Kanter, M. L. Funderburgh, M. M. Mann, Y. Du and J. L. Funderburgh. The Journal of biological chemistry (2007) 282(17): 12475-12483, PMCID 2912154.

Research Interests

Corneal Cell Biology Laboratory

Research Grants

NIH Grant R01 EY09360
Proteoglycans In Normal and Scarred Corneas. 4/1/2008-3/31/2013 (years 16-20) TDC $1,250,000 Role: Principal Investigator. Effort: 40%

Jules and Doris Stein Professorship , Research to Prevent Blindness Inc. 1/1/00-06/30/2010 TDC $525,000. Role: Awardee. Effort: No percentage effort required.

NIH Grant 1R01EY016415 Stem Cells for Corneal Engineering (years 6-9) TDC $1,161,000, Dates 4/1/2011-3/31/2015. Role: Principal Investigator. Effort 40%.

OTERO grant “Bioengineering of Corneal Stroma” From the Center for Vision Restoration. TDC $70,000 9/1/2009-8/30/2011.

P30 EY08098-20 Hendricks (PI & Module Director) 04/01/89-03/31/14
NIH/NEI 
“Core Grant for Vision Research”
The Core Grant will provide support for the following centralized research modules: Hybridoma/Tissue Culture; Molecular Biology; Electronic Shop; and Morphology & Image Analysis