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Faculty
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Paul (Kip) R. Kinchington
Associate Professor of Ophthalmology, Molecular Genetics and Biochemistry
The Campbell Laboratory for Infectious Eye Diseases
Ph.D.
Eye & Ear Institute
203 Lothrop Street, Room 1016
Pittsburgh, PA 15213
Phone: 412-647-6319
Email: kinchingtonp@upmc.edu
Fax: 412-647-5880
Faculty Research Interests
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 Research Interests
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Paul (Kip) Kinchington, Ph. D. heads the molecular herpesvirus unit of the Campbell Laboratory of Infectious Diseases in The Department of Ophthalmology. He has research programs studying three important viruses that cause common infectious ocular diseases, namely Varicella zoster virus (VZV), the cause of shingles; herpes simplex virus (HSV), the cause of much of the infectious disease-related corneal blindness seen in the US; and adenovirus, which causes ‘pink eye’, The main objective of the work studying VZV and herpes zoster is to characterize protein functions that could be targeted for antiviral strategy development, particularly those that influence the ability of the virus to interact with the host cell. This work has recently extended into studies aimed at investigating why VZV causes severe pain and post herpetic neuralgia which are the biggest complications of Shingles disease. A second program has developed in collaboration with Dr Robert Hendricks to study HSV and molecular aspects of its latency in the neuron, with a view to developing novel vaccines that not only prevent primary infections but also enhance the immunity to prevent reactivation and recurrent diseases, which cause corneal blindness. The work on adenoviruses is aimed at developing animal models to assess antivirals that could limit pink eye and its spread.
Dr Kinchington is also director of Molecular Biology facilities in the Department of Ophthalmology, which is an NEI funded and sponsored core facility to aid the programs of all vision researchers in Pittsburgh.
For more information, please follow these links:
Molecular Virology and Microbiology Program
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Education
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- Univ. of Leeds, Leeds, England 1983, Microbiology, Ph.D.
- Univ. of Leeds, Leeds, England 1979, Microbiology, B.Sc.(Honors) 1983-1990
- Uniformed Services University of The Health Sciences, Bethesda MD Post docotoral fellowship (1983-1986)
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Academic Affiliation
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POSITIONS HELD
1986-1990 Research Instructor, Dept. of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, MD
1991-1996 Assistant Professor, Dept. of Ophthalmology, University of Pittsburgh School of Medicine, and Dept. of Molecular Genetics & Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA
1996-present Associate Professor, Depts. of Ophthalmology and of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA.
Director, Molecular Biology Module
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Publications
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SELECT RECENT PUBLICATIONS
Decman V, Kinchington PR, Harvey SA, Hendricks RL. Gamma interferon can block herpes simplex virus type 1 reactivation from latency, even in the presence of late gene expression. J Virol. 2005 Aug;79(16):10339-47
Hood C, Cunningham AL, Slobedman B, Arvin AM, Sommer MH, Kinchington PR, Abendroth A. Varicella-zoster virus ORF63 inhibits apoptosis of primary human neurons. J Virol. 2006 Jan;80(2):1025-31
Eisfeld AJ Turse, S.E, S. A. Jackson, E. C Lerner and P. R. Kinchington. Phosphorylation of the varicella-zoster virus (VZV) major transcriptional regulatory protein IE62 by the VZV open reading frame 66 protein kinase. J Virol. 2006 Feb;80(4):1710-23
Garry EM, Delaney A, Anderson HA, Sirinathsinghji EC, Clapp RH, Martin WJ, Kinchington PR, Krah DL, Abbadie C, Fleetwood-Walker SM. Varicella zoster virus induces neuropathic changes in rat dorsal root ganglia and behavioral reflex sensitisation that is attenuated by gabapentin or sodium channel blocking drugs. Pain. 2005 Nov;118(1-2):97-111
R.P. Kowalski, P. Thompson, P. R. Kinchington and Y. J. Gordon. The Evaluation of the SmartCycler® II System for the Real-Time Detection of Viruses and Chlamydia from Ocular Specimens Archives of Ophthalmology, 2006 Aug;124(8):1135-9
S. Hasnie, J. Breuer S. Parker, V. Wallace, J. Blackbeard, I. Lever, P.R. Kinchington, A. H. Dickenson , T. Pheby, A. S. C. Rice. Further characterisation of a rat model of varicella zoster virus (VZV)-associated pain: relationship between mechanical hypersensitivity and anxiety-related behaviour; and the influence of analgesic drugs Neuroscience. 2007 Feb 23;144(4):1495-508
G.M.G.M. Verjans, R. Q. Hintzen, J.M. van Dun, A.Poot, J.C. Milikan,J. D. Laman, A.W. Langerak, P.R. Kinchington and A.D.M.E. Osterhaus. Selective retention of herpes simplex virus specific T cells in latently infected human trigeminal ganglia. PNAS 2007 Feb 27;104(9):3496-501
JCM. Milikan, PR. Kinchington GS Baarsma, RWAM Kuijpers, ADME. Osterhaus and GMGM Verjans* Identification of Viral Antigens Recognized by Ocular Infiltrating T Cells from Patients with Varicella Zoster Virus Induced Uveitis, IOVS 2007 Aug;48(8):3689-97
V. Decman M. L. Freeman, P. R. Kinchington and R. L. Hendricks. 2005. Immune control of HSV-1 latency.Viral Immunol. 2005;18(3):466-73
Ramachandran S and P. R. Kinchington. 2007. Prophylactic and therapeutic Herpes simplex virus vaccines. Current Pharmacology and Therautics, 13(19):1965-73
Eisfeld, A.J., Yee, M.B. Erazo, E., Abenderoth, A and P. R. Kinchington. Downregulation of Class I Major Histocompatibility Complex Surface Expression by Varicella-Zoster Virus Involves Open Reading Frame 66 Protein Kinase Dependent and Independent Mechanisms. J. Virology 2007 81(17):9034-49
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Grants
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NEI RO1 EY015291 (Kinchington, PI)Gene expression in HSV-1 latency after corneal infection 03-01-2004 to 02-28-2008.
NEI R01 EY09397-08, Kinchington (PI) PENDING "Varicella Zoster Virus Regulatory Proteins"
To identify the mechanisms underlying the unusual and novel ability of the gene 66 protein kinase to exclude the IE62 protein from the nucleus, and if this involves nuclear export processes. The project also will determine the mechanisms by which the major regulatory protein IE62 in packaged into the virion. The third objective is to identify the underlying mechanisms by which VZV protein kinase contributes to immune evasion.
NEI P30 EY08098 Hendricks (PI) 04/01/04-03/31/09 Kinchington (Module Supervisor) "Core Grant For Vision Research" The Core Grant will provide support for the following centralized research modules: Hybridoma/Tissue Culture, Molecular Biology, Electronic Shop, and Morphology Module.
NEI R01 EY08227 Gordon (PI) 05/01/91 - 04/30/08 Kinchington (Co-Investigator) NIH/NEI "Experimental Pathogenesis & Therapy of Ocular Adenovirus"
NEI R01 EY9368-10 Funderburgh PI) 08/01/93-07/31/012 Kinchington (Co investigator
NIH/NEI "Proteoglycans in Normal and Scarred Corneas"
Research To Prevent Blindness
07/01/99-06/30/02 5% "RPB Lew R. Wasserman Merit Award" $55,000
RPB's Lew R. Wasserman Merit Award is designed with the intent to provide unrestricted grants
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Lab Personnel
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J-P. Vergnes (research associate)
Michael Yee (lab manager and research associate)
Katherine Davoli (research technician)
Svridya Ramachandran (MVM Graduate student)
Angie Erazo (MVM Graduate student)
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My Links
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Molecular Biology and Virology - Kinchington (http://www.gradbiomed.pitt.edu/mvm/faculty.asp?ID=68)
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